Elucidating mechanism cellular uptake

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In our study, we chose three anisotropic geometries, star, rod, and triangle, to investigate the shape effect on cellular uptake into RAW264.7 cells.We fabricated gold nanostars (GNSs), gold nanorods (GNRs) and gold nanotriangles (GNTs) with similar size and coated them with methylpolyethylene glycol (m PEG) to obtain a neutral surface charge for excluding the interference of other factors (Fig. As is typically observed, m PEGylation allowed the GNPs to be well dispersed in aqueous solution and reduced the toxicity of the stabilizing agent CTAB. did evaluate the cellular uptake of GNPs by RAW264.7, but they did not investigate the endocytosis pathways. 2A–C, the GNPs exhibited distinct color variations.The cellular internalization of these nanoparticles by RAW264.7 cells was analyzed, providing a parametric evaluation of the effect of shape.

2D), which corresponded to the transverse longitudinal plasmon resonance of the elongated tips. 2F, the GNTs had a major plasmon band at 635 nm corresponding to the in-plane band; the band at 575 nm was related to the byproducts of the gold nanoparticles.The cellular uptake of P-GNPs was higher at 24 h than at 8 h.The results suggested that nanoparticle shape played an important role in cellular uptake.The intracellular concentrations of gold after incubation for 4 h with the P-GNPs were below the detection limit of inductively coupled plasma atomic emission spectrometer (ICP-AES).At 8 h and 24 h, shape and time-dependent cellular uptake was observed (Fig. After incubation for 24 h, gold concentrations in the cells incubated with P-GNSs, P-GNRs, and P-GNTs were 0.154 ± 0.010 pg/cell, 0.814 ± 0.001 pg/cell, and 1.333 ± 0.038 pg/cell, respectively.

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